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  2. Laboratory instruments and accessories
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  4. IAC columns

Afla B - Immunoafinity columns for Fluorometer & HPLC (25/box)

Reference: V*G1003

a excludes preparation and extraction// Here’s How Simple AFLA B is to Use: Extract Sample Grind and weigh sample Add salt and methanol/water mixture Filter Dilute and Filter D

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Category: Laboratory instruments and accessories / Chromatography / IAC columns

excludes preparation and extraction//

Here’s How Simple AFLA B is to Use:

Extract Sample

  • Grind and weigh sample
  • Add salt and methanol/water mixture
  • Filter

Dilute and Filter

  • Dilute extract
  • Filter

Absorb and Elute

  • Pass filtrate over Afla B affinity column
  • Wash column with water
  • Elute aflatoxins with methanol and collect in a cuvette

Measure

  • Add developer and place cuvette into a calibrated fluorometer, or
  • Inject eluate into HPLC
  • Determine aflatoxin concentratio
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